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Start Your TrialBlastN is part of a suite of programs provided by NCBI for aligning query sequences against those present in a selected target database. This implementation is specific for the TBAS project, and offers access only to that projects's databases. Please contact us if you have questions about our implementation and use cases.
tool=BLASTN_
input.query_ - Primary Input File
vparam.runtime_ - Float - Maximum Hours to Run (click here for help setting this correctly)
vparam.use_epa_ - Switch - User can specify whether or not they are using EPA Evolutionary Placement algorithm)
vparam.nucleotid_db_ - Excl - Sets the database that will be used. UNITE_public_tbas.expt; unaligned_its.expt; unaligned_lsu.expt; unaligned_its_lsu.expt; unaligned_ssu.expt; unaligned_mitssu.expt; unaligned_rpb1.expt; unaligned_rpb2.expt; sh_gr_28.06.2017.expt NOTE: unaligned databases cannot be used when use_epa=1
vparam.output_fmt_ - Excl - Sets the outputformat flag. 0, 1: query-anchored showing identities; 2: query-anchored no identities; 3: flat query-anchored, show identities; 4: flat query-anchored, no identities; 5: XML Blast output; 6: tabular; 7: tabular with comment lines; 8: text ASN.1; 9: binary ASN.1; 10: comma-separated value; 11: blast archive format
vparam.max_target_sequences_ - Integer - Number of aligned sequences to keep (default=500)